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5-fold larger activity than did the WT (determine 3a). Immunoblotting confirmed that the expression amounts of mutant Aph1 and PS1 ended up much like stages within the WT. These benefits counsel that γ-secretase with these two Aph1 mutations show enhanced protease exercise for APP.
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Aph1 mutants Energetic during the absence of NCT map to transmembrane domains. Mutations in double or one mutants are indicated within the product Together with the 7 transmembrane domains (TMDs) of Aph1. Mutations Lively as one mutants are demonstrated as Main mutations; mutations Lively only in combination are revealed click here as secondary mutations.
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